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KMID : 0381120210430101157
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Genes and Genomics
2021 Volume.43 No. 10 p.1157 ~ p.1165
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MiR-137 inhibits the proliferation, invasion and migration of glioma via targeting to regulate EZH2
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Gu Jingshun
Wang Juntong
You Aiwu
Li Jun
Zhang Yuyan
Rao Guomin
Ge Xuehua
Zhang Kun
Liu Xuan
Wang Dongchun
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Abstract
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Background: Gliomas are common malignant tumors in the nervous system, known for poor prognosis and low survival rate.
Objective: This study aims to explore functions of miR-137 in glioma progression and identify messenger RNAs (mRNA) regulated by miR-137, which provides new ideas for further exploration of glioma therapeutic targets.
Methods: Gene expression data were downloaded from the Cancer Genome Atlas database, and abnormally expressed miRNAs and mRNAs in glioma were analyzed. The expression of genes in 20 pairs of clinical tissue samples and glioma cell lines were detected through qRT-PCR, and the expression of proteins was detected through Western blot. Changes in cell proliferative level after transfection were detected via CCK8 assay, and changes in cell migratory and invasive abilities were detected by Transwell assay. Besides, dual-luciferase reporter assay was employed to testify binding relationship between two genes.
Results: Our study found that miR-137 was significantly and lowly expressed in glioma tissue and cell lines, and the prognoses of glioma patients with highly expressed miR-137 were more optimistic. Overexpressed miR-137 could remarkably inhibit proliferative, invasive and migratory abilities of glioma cells U87, while transfection of miR-137 inhibitor presented an opposite effect. Additionally, EZH2 was a direct target of miR-137 and overexpressed EZH2 effectively reversed the effect of miR-137 on glioma proliferation and migration.
Conclusions: Our study found that miR-137 could suppress the proliferation, invasion and migration of glioma cells through regulating the expression of EZH2. So far, we have found a novel regulatory pair that influences glioma progression, providing a basis for further development of new therapeutic strategies.
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KEYWORD
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Glioma, miR-137, EZH2, Proliferation, Migration, Invasion
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